Time-dependent association between platelet-bound fibrinogen and the Triton X-100 insoluble cytoskeleton
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Time-dependent association between platelet-bound fibrinogen and the Triton X-100 insoluble cytoskeleton.
Previous studies indicated a correlation between the formation of EDTA-resistant (irreversible) platelet-fibrinogen interactions and platelet cytoskeleton formation. The present study explored the direct association of membrane-bound fibrinogen with the Triton X-100 (Sigma Chemical Co, St Louis, MO) insoluble cytoskeleton of aspirin-treated, gel-filtered platelets, activated but not aggregated ...
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Triton X-100 solubilizes membranes of PC12 cells and leaves behind a nucleus and an array of cytoskeletal filaments. Nerve growth factor (NGF) receptors (10% of those found in intact cells) are associated with this Triton X-100-insoluble residue. Two classes of NGF receptors are found on PC12 cells which display rapid and slow dissociating kinetics. Although rapidly dissociating binding is pred...
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When ‘25l-labeled blood platelets were lysed with an equal volume of 2% Triton X-100, 10 mM EGTA, and 100 mM Tris, pH 7.4. the Triton-insoluble residue (“cytoskeleton”) contained about 1 1 % of the platelet protein. predominantly actin and actin-binding protein, 3.5% of the platelet radioactivity, and less than 2% of the platelet lipid phosphorus. Radioautographs of polyacrylamide gels of this ...
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When ‘25l-labeled blood platelets were lysed with an equal volume of 2% Triton X-100, 10 mM EGTA, and 100 mM Tris, pH 7.4. the Triton-insoluble residue (“cytoskeleton”) contained about 1 1 % of the platelet protein. predominantly actin and actin-binding protein, 3.5% of the platelet radioactivity, and less than 2% of the platelet lipid phosphorus. Radioautographs of polyacrylamide gels of this ...
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The plasma membrane proteins of Dictyostelium discoideum were characterized with respect to their partitioning into the Triton-insoluble cytoskeleton fraction of the cell during concanavalin A-induced capping. Two fractions of plasma membrane-associated concanavalin A were identified; one that immediately associated with the cytoskeleton fraction via cell surface glycoproteins, and one that par...
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ژورنال
عنوان ژورنال: Blood
سال: 1991
ISSN: 0006-4971,1528-0020
DOI: 10.1182/blood.v77.3.508.508